a Record control number 000253614700006

a ISSN 1661-5425

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QL955

b Item number .M76 2007

a Personal name Fahrioglu, U.

h Medium Electronic Document

a Title mRNA expression analysis and the molecular basis of neonatal testis defects in Dmrt1 mutant mice .

c Statement of responsibility, etc Umut Fahrioglu, M. W.Murphy, D. Zarkower, V. J. Bardwell.

c Date of publication, distribution, etc 2007.

b Name of publisher, distributor, etc Karger,

a Summary, etc Transcriptional regulators containing the DM domain DNA binding motif have been found to control sexual differentiation in a diverse group of metazoan animals including vertebrates, insects, and nematodes, suggesting that these proteins may comprise a very ancient group of sexual regulators. Dmrt1, 1 of 7 mammalian DM domain genes, is essential for several aspects of testicular differentiation in mice. The Dmrt1 mutant phenotype becomes apparent shortly after birth, and culminates in severe testicular dysgenesis. To better understand the roles of Dmrt1 in testicular development we have performed a more detailed analysis of its mutant phenotypes, and we have used mRNA expression profiling to identify genes misregulated in the neonatal Dmrt1 mutant testis. We find that Dmrt1 mutant germ cells fail to undergo several of the normal postnatal events of germ cell development, including radial movement, mitotic proliferation, differentiation into spermatogonia, and initiation of meiosis, and they die by P14. During this period Dmrt1 mutant Sertoli cells fail to polarize and form tight junctions, and fail to cease proliferation, eventually filling the seminiferous tubules. Expression profiling at P1 and P2 in Dmrt1 mutant testes indicates defects in several important testicular signaling pathways (Gdnf, retinoic acid, TGF beta, FSH), and detects elevated expression of the pluripotency marker Stella/Dppa3/Pgc7, providing insight into the molecular basis of Dmrt1 testis defects. This work also identifies a number of new candidate testicular regulators for further investigation. Copyright (C) 2007 S. Karger AG, Basel.

a Topical term or geographic name as entry element Developmental biology

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element DM domain

a Topical term or geographic name as entry element Dmrt1

a Main entry heading Sexual Development

g Relationship information 2007, Vol 1 Issue 1, p42-58

x International Standard Serial Number 16615425

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1159/000096238